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The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro

Received: 1 May 2018     Published: 23 May 2018
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Abstract

To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.

Published in Science Discovery (Volume 6, Issue 1)
DOI 10.11648/j.sd.20180601.16
Page(s) 35-42
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Human Umbilical Cord Blood Plasma, Immune, Exosome, Treg Cells

References
[1] Powrie F, Read S, Mottet C, et al. Control of immune pathology by regulatory T cells. [J]. Current Opinion in Immunology, 1998, 10(6):649-655.
[2] Shevach E M. Regulatory T cells in autoimmmunity*.[J]. Annual Review of Immunology, 2000, 18(1):423-449.
[3] Sakaguchi S. Regulatory T cells: key controllers of immunologic self-tolerance.[J]. Cell, 2000, 101(5):455-458.
[4] Shevach E M. CD4+ CD25+ suppressor T cells: more questions than answers.[J]. Nature Reviews Immunology, 2002, 2(6):389-400.
[5] Sakaguchi, S., N. Sakaguchi, M. Asano, M. Itoh, and M. Toda. 1995. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases..[J]. Journal of Immunology, 1995, 155(3):1151-64.
[6] Cools N, Ponsaerts P, Van Tendeloo V F, et al. Regulatory T Cells and Human Disease[J]. Clinical & Developmental Immunology, 2015, 2007(1740-2522):89195.
[7] Kondĕlková K, Vokurková D, Krejsek J, et al. Regulatory T cells (TREG) and their roles in immune system with respect to immunopathological disorders[J]. Acta Medica, 2010, 53(2):73.
[8] Safinia N, Sagoo P, Lechler R, et al. Adoptive regulatory T cell therapy: challenges in clinical transplantation[J]. Current Opinion in Organ Transplantation, 2010, 15(4):427.
[9] 江淑芳,史春梦.脐带血免疫学特性的研究进展[J].免疫学杂志, 2002,18(b06):149-152。
[10] Théry C, Zitvogel L, Amigorena S. Exosomes: composition, biogenesis and function[J]. Nature Reviews Immunology, 2002, 2(8):569.
[11] Lobb R J, Becker M, Wen S W, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma[J]. J Extracell Vesicles, 2015, 4:27031.
[12] Sódar B W, Ágnes Kittel, Pálóczi K, et al. Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection[J]. Scientific Reports, 2016, 6:24316.
[13] Goetzl E J, Boxer A, Schwartz J B, et al. Altered lysosomal proteins in neural-derived plasma exosomes in preclinical Alzheimer disease.[J]. Neurology, 2015, 85(1):40.
[14] Vicencio J M, Yellon D M, Sivaraman V, et al. Plasma exosomes protect the myocardium from ischemia-reperfusion injury.[J]. Journal of the American College of Cardiology, 2015, 65(15):1525-1536.
[15] Caby MP, Lankar D, VincendeauScherrer C, et al. Exosomal-like vesicles are present in human blood plasma.[J]. International immunology, 2005, 17(7):879.
[16] Jia R, Li J, Rui C, et al. Comparative Proteomic Profile of the Human Umbilical Cord Blood Exosomes between Normal and Preeclampsia Pregnancies with High-Resolution Mass Spectrometry.[J]. Cellular Physiology & Biochemistry International Journal of Experimental Cellular Physiology Biochemistry & Pharmacology, 2015, 36(6):2299-306.
[17] Yin Hu, Shan-Shan Rao, Zhen-Xing Wang, et al. Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function:[J]. Theranostics, 2018, 8(1):169.
[18] 李丹.血浆Exosomes生物学特征及血浆Exosomes对巨噬细胞功能影响的初步研究[D].复旦大学, 2011。
[19] Colombo M, Raposo G, Théry C. Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles[J]. Annual Review of Cell & Developmental Biology, 2014, 30(1):255.
[20] 李晓,刘玲英,柴家科.外泌体的生物学特性及临床应用的研究进展[J].解放军医学院学报,2015(10):1042-1044。
[21] Sun J, Yao Y, Chen L J, et al. Studies on biological properties and effects of anti-lymphoma of lymphoma cell-derived exosomes[J]. Journal of Practical Medicine, 2012.
[22] Robbins P D, Morelli A E. Regulation of immune responses by extracellular vesicles.[J]. Nature Reviews Immunology, 2014, 14(3):195.
[23] Mcgovern N, Shin A, Low G, et al. Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2[J]. Nature, 2017, 546(7660):662.
[24] 丛秀丽,赵启明.免疫系统与机体衰老[J].中国美容医学,2017, 26(1):16-20。
[25] 丁妍,卢智勇,袁雅红,等.脐血浆与胎牛血清培养人脐带间充质干细胞的比较[J].生物医学工程学杂志,2013(6):1279-1282。
Cite This Article
  • APA Style

    Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, et al. (2018). The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Science Discovery, 6(1), 35-42. https://doi.org/10.11648/j.sd.20180601.16

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    ACS Style

    Weiyi Xia; Zheng Ye; Hailing Li; Doulathunnisa Jaffar Ali; Jiahua Ding, et al. The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Sci. Discov. 2018, 6(1), 35-42. doi: 10.11648/j.sd.20180601.16

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    AMA Style

    Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, et al. The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Sci Discov. 2018;6(1):35-42. doi: 10.11648/j.sd.20180601.16

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  • @article{10.11648/j.sd.20180601.16,
      author = {Weiyi Xia and Zheng Ye and Hailing Li and Doulathunnisa Jaffar Ali and Jiahua Ding and Bo Sun and Zhongdang Xiao},
      title = {The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro},
      journal = {Science Discovery},
      volume = {6},
      number = {1},
      pages = {35-42},
      doi = {10.11648/j.sd.20180601.16},
      url = {https://doi.org/10.11648/j.sd.20180601.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20180601.16},
      abstract = {To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro
    AU  - Weiyi Xia
    AU  - Zheng Ye
    AU  - Hailing Li
    AU  - Doulathunnisa Jaffar Ali
    AU  - Jiahua Ding
    AU  - Bo Sun
    AU  - Zhongdang Xiao
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    PY  - 2018
    N1  - https://doi.org/10.11648/j.sd.20180601.16
    DO  - 10.11648/j.sd.20180601.16
    T2  - Science Discovery
    JF  - Science Discovery
    JO  - Science Discovery
    SP  - 35
    EP  - 42
    PB  - Science Publishing Group
    SN  - 2331-0650
    UR  - https://doi.org/10.11648/j.sd.20180601.16
    AB  - To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.
    VL  - 6
    IS  - 1
    ER  - 

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Author Information
  • State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

  • State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

  • Zhongda Hospital, Department of Obstetrics & Gynecology,Southeast University, Nanjing, China

  • State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

  • Zhongda Hospital, Department of Hematology, Key Deptment Jiangsu Medicine, Southeast University, Nanjing, China

  • State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

  • State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

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